| Popis: |
Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCRRFLP) and Single Nucleotide polymorphism (SNP) techniques were used to study the association between bone morphogenetic protein receptor IB (BMPR IB) gene polymorphism with litter size trait and kids growth. Forty four Female goats were precisely selected according to their litter size and kids growth. PCR amplification of 190 bp of the BMPR-IB gene was genotyped in all goats and sequenced only in those produced the highest and lowest litter size and kids growth. Restriction analysis of PCR-RFLP using Ava II and Hind III of the BMPR-IB gene (190-bp) do not produce restriction fragments. By DNA sequencing, eight single nucleotide polymorphisms (SNPs) at seven different positions were obtained. Furthermore, with translation of SNPs to corresponding amino acids, change of six amino acids in three female goats were obtained as the flowing, Baladi goat with high litter size, glutamic acid (E) changed to aspartic acid (P) and isoleucine (I) changed to valine (V). In high litter size, Zaraibi goat, valine (V) changed to leucine acid (L) and glutamine (Q) changed to histidine (H). In another Zaraibi Goat with high litter size valine (V) changed to leucine acid (L) and threonine (T) changed to proline (P). These findings can be used in a marker-assisted selection (MAS) for selection for high litter size trait in goats. There are negative relationships in most goats between SNPs in BMPR IB gene and relative growth gain (RGG). |
