Technetium-labeling of monoclonal antibodies with functionalized BATOs: 2. TcCl(DMG)3CPITC (CPITC=carboxyphenylisothiocyanate) labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab')2
| Autor: | R. M. Sharkey, William C. Eckelman, K. Ramalingam, David P. Nowotnik, K. E. Linder, Rama K. Narra, F. Yost, M. D. Wen, A. D. Nunn |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pharmacology
medicine.drug_class Organic Chemistry Biomedical Engineering Substituent Pharmaceutical Science chemistry.chemical_element Bioengineering Monoclonal antibody Technetium Adduct chemistry.chemical_compound Dimethylglyoxime chemistry Reagent Isothiocyanate medicine Boronic acid Biotechnology Nuclear chemistry |
| Zdroj: | Bioconjugate Chemistry. 2:407-414 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | BATO (boronic acid adduct of technetium dioximes) complexes, TcCl(dioxime)3BR, were prepared in which the boron substituent (R) was the protein-reactive 2-carboxy-4-phenyl isothiocyanate (CPITC). The 99Tc complexes, where the dioxime was either dimethylglyoxime (DMG) or cyclohexanedione dioxime (CDO), were prepared and characterized. The 99mTc complex TcCl(DMG)3CPITC was prepared from a freeze-dried kit and used to label B72.3 (anti-TAG.72) and NP-4 (anti-CEA) whole antibodies, and the NP-4 F(ab')2 fragment. SDS-PAGE electrophoresis indicated that the labeling reagent was strongly bound to antibody. The labeled antibodies displayed high binding to affinity columns and good tumor uptake in GW39 tumor-bearing mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|