Affinity column clean‐up for the analysis of fumonisins and their hydrolysis products in corn

Autor:	J. L. Richard, Chris M. Maragos, Glenn A. Bennett
Rok vydání:	1997
Předmět:	Fusarium Chromatography biology Immunology Extraction (chemistry) food and beverages Contamination biology.organism_classification Clean-up Hydrolysis chemistry.chemical_compound chemistry Affinity chromatography Methanol Mycotoxin Agronomy and Crop Science Food Science
Zdroj:	Food and Agricultural Immunology. 9:3-12
ISSN:	1465-3443 0954-0105
Popis:	Fumonisins, toxic metabolites of certain Fusarium molds, can be found in corn and corn‐based foods at levels sufficient to cause disease in livestock. Widely used methods of analysis involve organic extraction followed by isolation with either C18 or strong anion exchange columns. An affinity column procedure that simultaneously isolated both intact and hydrolyzed fumonisins from corn is reported. The columns were prepared using two monoclonal antibodies with differing specificities, the first (P2A5–3‐F3) bound intact fumonisins, while the second (P2F11–3‐H7) bound their hydrolysis products. Corn samples were extracted with methanol/phosphate buffer (80:20, v:v) and the extract was diluted and applied to the affinity column. The recoveries of FB1 from corn spiked with 0.5–8.0 ppm averaged 82%. The recoveries of HFB1 over the range 0.25–1.25 ppm averaged 102%. Affinity columns capable of binding both intact and hydrolyzed fumonisins allow for the analysis of both types of toxins simultaneously from a singl...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02ffcb57f5ab2c374d522fad43c363b1 https://doi.org/10.1080/09540109709354929 Zobrazit plný text záznamu