Activation of NF-kB transcription factor in hepatoma cell cultures by carcinogenic polycyclic aromatic hydrocarbons

Autor:	V. A. Kobliakov, M. S. Volkov
Rok vydání:	2011
Předmět:	biology Stimulation Cell Biology Aryl hydrocarbon receptor medicine.disease_cause Molecular biology Hep G2 chemistry.chemical_compound chemistry Biochemistry Gene expression biology.protein medicine Pyrene Carcinogenesis Transcription factor Carcinogen
Zdroj:	Cell and Tissue Biology. 5:378-382
ISSN:	1990-5203 1990-519X
DOI:	10.1134/s1990519x11040122
Popis:	The activation of NF-kB transcription factor produced by carcinogenic polycyclic aromatic hydrocarbons (PAHs) benzo/a/pyrene (BP) and 3-methylcholanthrene (MC) in cells with (human hepatoma HepG2) or without (rat hepatoma G27) the expression of the Ah receptor has been studied. The NF-kB activity was estimated by NF-kB-dependent I-kB gene expression and by the transcriptional activity of the luciferase reporter gene under the NF-kB-sensitive promoter. BP and MC weakly enhanced the NF-kB activity in proliferating Hep G2 cells, whereas the stimulation of NF-kB activity was significantly higher in resting cells. The activation of NF-kB by BP and MC in hepatoma G27 cells was significantly higher than in Hep G2 cells in both proliferating and resting cells. The role of Ah receptor in the PAH activation of NF-kB transcription factor is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02913d32e3619d3578f0bc029ca62e03 https://doi.org/10.1134/s1990519x11040122 Zobrazit plný text záznamu Full text from SpringerLink