Heterozygosity for cervid S138N polymorphism results in subclinical CWD in gene-targeted mice and progressive inhibition of prion conversion

Autor: Maria I. Arifin, Lech Kaczmarczyk, Doris Zeng, Samia Hannaoui, Chi Lee, Sheng Chun Chang, Gordon Mitchell, Debbie McKenzie, Michael Beekes, Walker Jackson, Sabine Gilch
Rok vydání: 2023
Předmět:
Zdroj: Proceedings of the National Academy of Sciences. 120
ISSN: 1091-6490
0027-8424
DOI: 10.1073/pnas.2221060120
Popis: Prions are proteinaceous infectious particles that replicate by structural conversion of the host-encoded cellular prion protein (PrP C ), causing fatal neurodegenerative diseases in mammals. Species-specific amino acid substitutions (AAS) arising from single nucleotide polymorphisms within the prion protein gene ( Prnp ) modulate prion disease pathogenesis, and, in several instances, reduce susceptibility of homo- or heterozygous AAS carriers to prion infection. However, a mechanistic understanding of their protective effects against clinical disease is missing. We generated gene-targeted mouse infection models of chronic wasting disease (CWD), a highly contagious prion disease of cervids. These mice express wild-type deer or PrP C harboring the S138N substitution homo- or heterozygously, a polymorphism found exclusively in reindeer ( Rangifer tarandus spp. ) and fallow deer ( Dama dama ). The wild-type deer PrP-expressing model recapitulated CWD pathogenesis including fecal shedding. Encoding at least one 138N allele prevented clinical CWD, accumulation of protease-resistant PrP (PrP res ) and abnormal PrP deposits in the brain tissue. However, prion seeding activity was detected in spleens, brains, and feces of these mice, suggesting subclinical infection accompanied by prion shedding. 138N-PrP C was less efficiently converted to PrP res in vitro than wild-type deer (138SS) PrP C . Heterozygous coexpression of wild-type deer and 138N-PrP C resulted in dominant-negative inhibition and progressively diminished prion conversion over serial rounds of protein misfolding cyclic amplification. Our study indicates that heterozygosity at a polymorphic Prnp codon can confer the highest protection against clinical CWD and highlights the potential role of subclinical carriers in CWD transmission.
Databáze: OpenAIRE
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