Serotonin, GnRH-A, and dopamine interaction in the control of in vivo luteinizing hormone release in Prussian carp (Carassius gibelio Bloch) at the time of gonad recrudescence
| Autor: | G. Gosiewski, Miroslawa Sokolowska-Mikolajczyk, Magdalena Socha, D. Gajdzinski |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Prussian carp endocrine system medicine.medical_specialty Gonad biology Dopamine antagonist biology.organism_classification 03 medical and health sciences 030104 developmental biology Endocrinology Pimozide medicine.anatomical_structure Dopamine hemic and lymphatic diseases Internal medicine medicine Carassius Animal Science and Zoology Serotonin Luteinizing hormone hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Czech Journal of Animal Science. 60:45-51 |
| ISSN: | 1805-9309 1212-1819 |
| DOI: | 10.17221/7973-cjas |
| Popis: | The effects of serotonin (5-HT), GnRH analogue (D-Ala 6 LHRH, GnRH-A) and dopamine antagonist - pimozide (PIM), on luteinizing hormone (LH) release in mature Prussian carp female ( Carassius gibelio Bloch) were examined at the time of gonad recrudescence. Fish were intraperitoneally injected with 5-HT (10 mg/kg), GnRH-A (20 μg/kg) or PIM (5 mg/kg) or the combinations: 5-HT+GnRH-A, 5-HT+PIM, 5-HT+GnRH-A+PIM. Before the injection and 3, 6, 12 or 24 h after treatment blood samples were collected for LH levels determination by ELISA method. The analysis of LH concentrations, expressed as the percentage of pre-treatment, showed that serotonin alone had no influence on the spontaneous LH release, however the additive effects of serotonin and GnRH-A was observed. Serotonin potentiated the GnRH-A-stimulated LH release and potentiated also the effect of PIM. Extremely strong response to PIM and also to the combination with GnRH-A masked the participation of serotonin in the process of LH release in fish with recrudescing gonads. The interaction of serotonin GnRH-A and PIM in the control of LH release is discussed. |
| Databáze: | OpenAIRE |
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