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Bin Shi,1 Aixia Sun,2 Xiaorui Zhang3 1Department of Gastrointestinal Surgery, Liaocheng People’s Hospital of Taishan Medical University, Liaocheng, Shandong Province, China; 2Department of Clinical Laboratory, Liaocheng People’s Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong Province, China; 3Department of Health, Liaocheng People’s Hospital of Taishan Medical University, Liaocheng, Shandong Province, China Purpose: In cytokine-induced killer (CIK) cell therapy, the phenotypes and the numbers of CIK cells have a great influence on the therapeutic effects. This study aimed to investigate the effects of different ex vivo cell culture methods on the proliferation and cytotoxicity of CIK cells that were obtained from gastric cancer patients.Patients and methods: CIK precursor (Pre-CIK) cells were collected by either hydroxyethyl starch (HES) sedimentation (HES method, unpurified group) or Ficoll-Hypaque density gradient centrifugation (Ficoll method, purified group). Cell number, collection time, and morphology of Pre-CIK cells in the two groups were determined. The proliferation ability, cytokines, phenotypes, and cytotoxicity of CIK cells in the two groups were evaluated ex vivo and in vivo.Results: In this study, the number of Pre-CIK cells in the unpurified group was significantly higher than that in the purified group (P |
