Using RAPD techniques to check the genetic stability of Erigeron nanus schur regenerants in the ex situ conservation context
| Autor: | Rodica CATANĂ, Matilda CIUCĂ, Irina HOLOBIUC |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Analele Universitatii din Oradea: Fascicula Biologie, Vol TOM XVII, Iss 2, Pp 230-234 (2010) |
| ISSN: | 1844-7589 1224-5119 |
| Popis: | In this study we intended to check the genetic stability of micropropagated shoots obtained through indirect organogenesis of a rare, Carpathian endemic Erigeron nanus Schur plant species using the RAPD techniques. Our study is joining to the Convention on Biological Diversity’s 2010 target, which consists in the reduction of the current rate of biodiversity loss, trying to check the genetic stability of micropropagated plants at Erigeron nanus Schur for the conservative purpose. An in vitro propagation protocol through indirect organogenesis has been developed for multiplication of Erigeron nanus Schur and has been used continuously as an initial step for the production and maintenance of shoot cultures. Reproducible RAPD patterns were obtained using 4 primers which showed monomorphic bands in the all samples tested. Despite of the pre-request conditions for somaclonal variation (indirect micropropagation pathway - via calli), duration of in vitro culture (more than 12 months), in vitro stress due to unnatural conditions, the tested primers showed any differences in the RAPD pattern of the regenerants. |
| Databáze: | OpenAIRE |
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