COMPARISON OF LIPOLYTIC ACTIVITY OF Sporothrix schenckii STRAINS UTILIZING OLIVE OIL-RHODAMINE B AND TWEEN 80
| Autor: | Mariana Carissimi, Thais Furtado de Souza, Valeriano Antonio Corbellini, Maria Lúcia Scroferneker |
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| Jazyk: | portugalština |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Tecno-Lógica, Vol 11, Iss 1-2, Pp 33-36 (2007) |
| ISSN: | 1982-6753 1415-6229 |
| Popis: | The aim of this work was to identify the lipase activity of Sporothrix schenckii strains using Rhodamine B as an indicator oftriglyceride hydrolysis (triolein from olive oil) and to compare these results with the use of Tween 80 as substrate. Free fatty acidsreleased from lipase activity were detected on agar plate complexed with Rhodamine B, when exposed to UVA light, through orangefluorescence and by precipitation haloes on Tween 80 agar. There were differences in lipolytic activity among strains and between themedia utilized. The highest lipase activity (orange fluorescence) was observed on the biomass of 10 strains, with no fluorescent haloesaround the colonies, whereas only six strains showed large precipitation haloes. These plate assays are suitable for the detection oflipase-producing strains of S. schenckii in the yeast phase, but it is still unknown whether different enzymes act upon the substrates orwhether there are variations of the same enzyme with different specificities. |
| Databáze: | OpenAIRE |
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