INDUCTION OF APOPTOSIS IN B16 MELANOMA CELLS UNDER THE ACTION OF DRUG CONTAINING TUMOR NECROSIS FACTOR ALPHA AS A PART OF VIRUS-LIKE PARTICLES IN VITRO
| Autor: | E. A. Vyazovaya, L. R. Lebedev, E. D. Danilenko |
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| Jazyk: | ruština |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Sibirskij Onkologičeskij Žurnal, Vol 19, Iss 4, Pp 99-104 (2020) |
| ISSN: | 2312-3168 1814-4861 |
| Popis: | Aim: to evaluate the antitumor activity of the drug containing TNF-alpha and high-polymer double-stranded RNA (dsRNA) in the composition of virus-like particles (VLP-TNF-alpha) on B16-F10 melanoma cells. Material and Methods. Analysis of the anti-proliferative effect of VLP-TNF-alpha as well as its components, TNFalpha and dsRNA, was carried out using the MTT -test. Apoptosis of melanoma cells was assessed by flow cytofluorimetry with FITC-annexin V. Results. It was shown that the cytotoxic effect of the drug containing the combination of TNF-alpha and dsRNA on melanoma cells significantly exceeded the total cytotoxic effect of TNF-alpha or dsRNA alone (LD50 for combination drug was 0.05 μg/ml, TNF-alpha – 9.5 μg/ml, dsRNA>20 μg/ml). Conclusion. The drug containing TNF-alpha and dsRNA molecules may be a promising drug for the treatment of malignant tumors, including melanoma. |
| Databáze: | OpenAIRE |
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