Identification of novel adipokines in the joint.Differential expression in healthy and osteoarthritis tissues

Autor:	Conde Aranda, Javier, Scotece, M., Abella Cajigal, Vanesa, Gómez Bahamonde, Rodolfo, López López, Verónica, Villar, R., Hermida Sánchez, Miguel, Pino Mínguez, Jesús, Gómez-Reino Carnota, Juan Jesús, Gualillo ., Oreste
Rok vydání:	2015
Předmět:	Adult Aged 80 and over Membrane Glycoproteins Knee Joint Proteinase Inhibitory Proteins Secretory Gene Expression Middle Aged Osteoarthritis Knee CCN Intercellular Signaling Proteins Repressor Proteins Young Adult Adipokines Adipose Tissue Serpin E2 Humans Arthroplasty Replacement Knee Aged
Zdroj:	RUNA. Repositorio da Consellería de Sanidade e Sergas Servizo Galego de Saúde (SERGAS)
Popis:	OBJECTIVES: Emerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues. METHODS: Synovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52-85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23-53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively. RESULTS: All the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls. CONCLUSIONS: In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues. Xunta de Galicia/SERGAS Instituto de Salud Carlos III (ISCIII) Fundación Ramón Domínguez Ministerio de Educación de España
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::ead761b30b5f58bbfb1553d7e8cb6a12 http://hdl.handle.net/20.500.11940/8167 Zobrazit plný text záznamu