Associations of the -238(G/A) and -308(G/A) TNF-α Promoter Polymorphisms and TNF-α Serum Levels with the Susceptibility to Gastric Precancerous Lesions and Gastric Cancer Related to Helicobacter pylori Infection in a Moroccan Population
| Autor: | Bounder, Ghizlane, Jouimyi, Mohamed Reda, Boura, Hasna, Touati, Eliette, Michel, Valérie, Badre, Wafaa, Jouhadi, Hassan, Kadi, Maria, Eljihad, Meriem, Benomar, Hakima, Kettani, Anass, Lebrazi, Halima, Maachi, Fatima |
|---|---|
| Přispěvatelé: | Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP), Faculté des Sciences Ben M'sik [Casablanca], Université Hassan II [Casablanca] (UH2MC), Pathogenèse de Helicobacter, Institut Pasteur [Paris] (IP), CHU Ibn Rochd [Casablanca], This work is part of an approved thesis and it was supported by Pasteur Institute International Network [ACIP 2015-A-10-2015], We acknowledge the contributions of Our Patients Partners, and we ask God for them the recovery and health. Dr. Nadia El Gnaoui, Mrs. Maria Serdani, Mrs. Saida Moutahir, and Dr. Amal Oukkadi from the Laboratory of Histo-Cytopathology, Institut Pasteur du Maroc, Casablanca, Morocco. Dr. Meriem Khyati from Laboratory of Onco-Virology, Institut Pasteur du Maroc. Pr Nordine Habti from Faculty of Medicine and Pharmacy of Casablanca. Dr. Abdeljabbar Majd from Regional Transfusion Center of Casablanca., Institut Pasteur [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Cancer Prevention Asian Pacific Journal of Cancer Prevention, 2020, 21 (6), pp.1623-1629. ⟨10.31557/APJCP.2020.21.6.1623⟩ Asian Pacific Journal of Cancer Prevention, Asian Pacific Education Press Ltd., 2020, 21 (6), pp.1623-1629. ⟨10.31557/APJCP.2020.21.6.1623⟩ |
| ISSN: | 1513-7368 |
| DOI: | 10.31557/APJCP.2020.21.6.1623⟩ |
| Popis: | International audience; Objective: Helicobacter pylori (H. pylori) induces the production of tumor necrosis factor-alpha (TNF-α), which is closely related to a gastric epithelial injury. TNF-α gene polymorphism and TNF-α serum levels are associated with various malignant conditions. Identification of the ideal marker for gastric cancer (GC) is still the leading aim of several trials. Physio-pathological considerations of GC led us to investigate the association of two TNF-α promoter polymorphisms (-308G>A and -238G>A), and TNF-α serum levels with the susceptibility to gastric precancerous (PL) and GC.Methods: Patients suffering from gastric lesions (65 chronic gastritis, 50 PL, 40 GC) related to H. pylori infection , and 63 healthy controls (HC) were involved in this study. Individuals are genotyped by TNF-α gene promoter sequencing and TNF-α serum levels are measured by ELISA quantitative method.Results: Regarding TNF-α-308 G/A locus, we noticed higher risk for GC (OR=4.3, CI 1.5-11.9, p-value=0.005) and PL (OR=3.4, CI 1.2-9.2, p-value=0.01) for individuals with AA/GA genotypes compared to GG genotype. Concerning TNF-α-238 G/A locus, we noticed higher risk for GC (OR=5.9, CI 1.2-27.5, p-value=0.01) and PL (OR=4.8, CI 1.3-18, p-value=0.01) for individuals with GG genotype compared to AA/GA genotypes. We noticed that TNF-α serum levels have been increased together with gastric lesions severity. Moreover, TNF-α-308 and TNF-α-238 A alleles seemed to, respectively, upregulate and downregulate TNF-α serum levels.Conclusion: The TNF-α -308 A allele has a promotive effect for GC progression, whereas the TNF-α -238 A allele has a protective function against GC progression. High levels of TNF-α seemed to be associated with the aggressiveness of gastric lesions. TNF-α gene polymorphisms and TNF-α serum levels might be helpful to select those patients who are at high risk for GC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|