| Autor: |
Taghizadehghalehjoughi, Ali, Hacimuftuoglu, Ahmet, Cetin, Meltem, Kaplan, Afife Busra Ugur, Butuner, Selcuk, Taspinar, Numan, Mohammadzadeh, Maryam |
| Přispěvatelé: |
Uşak Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Nanomedicine Journal, Vol 6, Iss 1, Pp 35-42 (2019) |
| Popis: |
WOS: 000454742500005 Objective(s): The present study was designed to evaluate of Metformin/Irinotecan-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) effects on glutamate re-uptake time and receptor expression status in both glioblastoma multiforme (GBM) and cortex neuron cultures. The study was performed on glioblastoma cell line and primer cortex neuron. Materials and Methods: The re-uptake time and gene expression status of pure drugs and MET- or IRI-loaded-PLGA NPs on healthy neuron cells and U-87 MG cell line were investigated by using glutamate specific voltammetry electrodes technique and real time PCR. Results: Both MET and MET-PLGA NPs (1 and 2 mM) exhibited significant cytotoxicity on both U87MG and neuron cells. MET and MET-PLGA NPs (0.5 mM) showed lower cytotoxic effects on both cells. IRI and IRI-PLGA NPs (100 mu M) had significant cytotoxic effects on both cell lines. Conclusion: All drug-loaded NPs caused a significant reduction in glutamate reuptake time compared with free drugs, blank NPs and cancer cells control groups. Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach to treat GBM. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113S083] This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, Project Number: 113S083). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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