Comparison of Some Bacterial Identification Methods

Autor: GÜLAYDIN, Özgül, EKİN, İsmail Hakkı, ÖZTÜRK, Cihat, İLHAN, Ziya, ÖĞÜN, Erdal
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Volume: 3, Issue: 1 9-12
Turkish Journal of Veterinary Research
ISSN: 2602-3695
Popis: Objective: Inthis study, three different methods were compared for the identification ofsome Gram positive and Gram negative reference bacteria.Materials and Methods: Forthis purpose the identification accuracy rates of Enterococcus faecalis, Enterococcus faecium, Streptococcus agalactiae,Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonellatyphimurium, Serratia marcescens were analysed by conventional bacteriologicalmethods, commercial bacterial identification test kit (Microgen™ ID) and automatedbacteria identification system (BD Phoenix 100™).Results: As aresult of analyses, the identification accuracy rates of examined cultures were94.5%, 95.2%, 94.6%, 89.6%, 91.1%, 92.5%, 86.9%, 96.4% by conventionalbacteriological methods, 93.84%, 89.2%, 98.86%, 96.55%, 97.98%, 95.43%, 86.69%,92.39% by commercial bacterial identification test kit and 98%, 99%, 99%, 96%,96%, 97%, 99%, 98% by automated bacteria identification system, respectively.Conclusion: In comparison of methods, the identification accuracyvalues obtained from the automated system were higher than the other methods. Itwas concluded that automated identification systems that developed foraccurate, reliable and rapid identification of bacteria, could be used as analternative to conventional methods and commercial kits. In addition, it wasthought that it would be useful to evaluate the identification of biotypesobtained from clinical isolates by similar methods.
Databáze: OpenAIRE