Blocking variant surface glycoprotein synthesis alters ERES/ Golgi homeostasis in Trypanosoma brucei
| Autor: | Ooi, Cher-Pheng, Smith, Terry K., Gluenz, Eva, Wand, Nadina Vasileva, Vaughan, Sue, Rudenko, Gloria |
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| Přispěvatelé: | Wellcome Trust |
| Rok vydání: | 2018 |
| Předmět: |
Science & Technology
membrane metabolism UNFOLDED PROTEIN RESPONSE 0601 Biochemistry And Cell Biology SECRETORY PATHWAY DRUG TARGET Cell Biology POSTTRANSLATIONAL MODIFICATION Golgi biogenesis GPI-ANCHORED PROTEINS SPHINGOLIPID SYNTHESIS parasitic diseases ER exit site Trypanosoma brucei Life Sciences & Biomedicine BLOOD-STREAM-FORM AFRICAN TRYPANOSOMES PLANT GOLGI-APPARATUS INTRACELLULAR-TRANSPORT variant surface glycoprotein Developmental Biology |
| Popis: | The predominant secretory cargo of bloodstream form Trypanosoma brucei is variant surface glycoprotein (VSG), comprising ~10% total protein and forming a dense protective layer. Blocking VSG translation using Morpholino oligonucleotides triggered a precise pre-cytokinesis arrest. We investigated the effect of blocking VSG synthesis on the secretory pathway. The number of Golgi decreased, particularly in post-mitotic cells, from 3.5 +/- 0.6 to 2.0 +/- 0.04 per cell. Similarly, the number of endoplasmic reticulum exit sites (ERES) in post-mitotic cells dropped from 3.9 +/- 0.6 to 2.7 +/- 0.1 eight hours after blocking VSG synthesis. The secretory pathway was still functional in these stalled cells, as monitored using Cathepsin L. Rates of phospholipid and glycosylphosphatidylinositol-anchor biosynthesis remained relatively unaffected, except for the level of sphingomyelin which increased. However, both endoplasmic reticulum and Golgi morphology became distorted, with the Golgi cisternae becoming significantly dilated, particularly at the trans-face. Membrane accumulation in these structures is possibly caused by reduced budding of nascent vesicles due to the drastic reduction in the total amount of secretory cargo, that is, VSG. These data argue that the total flux of secretory cargo impacts upon the biogenesis and maintenance of secretory structures and organelles in T. brucei, including the ERES and Golgi. |
| Databáze: | OpenAIRE |
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