PRIKAZ IZRAŽAJA CRELD2 U EMBRIONALNOM I POSTNATALNOM BUBREGU DAB1-/- MIŠEVA

Autor:	Đurđević, Nikolina
Přispěvatelé:	Vukojević, Katarina, Mardešić, Snježana, Božić, Joško, Filipović, Natalija
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences. Cytology Histology and Embryology Mice CRELD2 protein mouse BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti. Citologija histologija i embriologija Mišji protein CRELD2 Gene Expression Bubreg Miševi Kidney Izražaj gena
Popis:	Objectives: The expression and localization of Creld2 in the nephrons of yotari (Dab1-/-) and wildtype (Dab1+/+) mice was analyzed to further develop its suggested importance in mammal kidneys overall, and its significance particularly in yotari mice nephrons. Materials and methods: Yotari and wildtype mice were sacrificed on the 13.5th embryonal day, and the 4th and 14th postnatal day. Paraffin embedded kidney tissue sections were analyzed by immunofluorescence using Creld2 antibodies. Kidney structures were then examined by fluorescence microscope. The percentages of positive cells in the different nephron structures were compared between yotari and wildtype mice and analyzed by a Kruskal-Wallis test, followed by a t-test. Results: The PCT of postnatal 14D wildtype nephrons showed a strong Creld2 expression signal in the cell cytoplasm and was the structure with the highest percentage of immunoreactive cells (95%) with significantly higher expressions compared to the yotari mice group (P Cilj: Analizirali smo izražaj i lokalizaciju Creld2 u nefronima yotari miševa (Dab1-/-) i divljeg tipa (Dab1+/+) kako bi se dalje utvrdio njegov predloženi značaj u bubrezima sisavaca općenito, kao i njegov značaj posebno u bubrezima yotari miševa. Materijali i metode: Yotari miševi i miševi divljeg tipa žrtvovani su 13.5-og embrionalnog dana, te četvrtog i četrnaestog postnatalnog dana. Parafinski rezovi bubrežnog tkiva analizirani su imunofluorescencijom pomoću Creld2 protutijela. Bubrežne strukture ispitivane su fluorescencijskim mikroskopom. Postotak pozitivnih stanica između svake skupine uspoređen je i analiziran pomoću Kruskal-Wallis testa i potom korištenjem t-testa. Rezultati: PCT 14 postnatalnih dana starog nefrona divljeg tipa pokazao je jaku ekspresiju Creld2 signala u staničnoj citoplazmi i bila je struktura sa najvećim postotkom imunoreaktivnih stanica (95%) sa signifikantno višom ekspresijom u usporedbi s grupom yotari miševa (P
Databáze:	OpenAIRE
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