Multispacer Sequence Typing Relapsing Fever Borreliae in Africa
| Autor: | Haitham, E., Gimenez, G., Sokhna, Cheikh, Bilcha, K. D., Ali, J., Barker, S. C., Cutler, S. J., Raoult, Didier, Drancourt, M. |
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| Přispěvatelé: | Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Gondar, University of Queensland [Brisbane], University of East London (UEL), ANR-08-MIEN-0004,BORETIC,Diagnostic et épidémiologie moléculaires des infections à Borrelia crocidurae(2008), INSB-INSB-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Genotyping
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:Public aspects of medicine Borrelia Relapsing fever Sequence analysis lcsh:RA1-1270 [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Ticks Blood [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Borrelia infection DNA sequence analysis |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, 2012, 6 (6), pp.e1652. ⟨10.1371/journal.pntd.0001652⟩ PLoS Neglected Tropical Diseases, Public Library of Science, 2012, 6 (6), pp.e1652. ⟨10.1371/journal.pntd.0001652⟩ PLoS Neglected Tropical Diseases, Vol 6, Iss 6, p e1652 (2012) |
| ISSN: | 1935-2727 1935-2735 |
| DOI: | 10.1371/journal.pntd.0001652⟩ |
| Popis: | International audience; Background: In Africa, relapsing fevers are neglected arthropod-borne infections caused by closely related Borrelia species. They cause mild to deadly undifferentiated fever particularly severe in pregnant women. Lack of a tool to genotype these Borrelia organisms limits knowledge regarding their reservoirs and their epidemiology.Methodology/Principal Findings: Genome sequence analysis of Borrelia crocidurae, Borrelia duttonii and Borrelia recurrentis yielded 5 intergenic spacers scattered between 10 chromosomal genes that were incorporated into a multispacer sequence typing (MST) approach. Sequencing these spacers directly from human blood specimens previously found to be infected by B. recurrentis (30 specimens), B. duttonii (17 specimens) and B. crocidurae (13 specimens) resolved these 60 strains and the 3 type strains into 13 species-specific spacer types in the presence of negative controls. B. crocidurae comprised of 8 spacer types, B. duttonii of 3 spacer types and B. recurrentis of 2 spacer types.Conclusions/Significance: Phylogenetic analyses of MST data suggested that B. duttonii, B. crocidurae and B. recurrentis are variants of a unique ancestral Borrelia species. MST proved to be a suitable approach for identifying and genotyping relapsing fever borreliae in Africa. It could be applied to both vectors and clinical specimens. |
| Databáze: | OpenAIRE |
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