| Autor: |
Ahmed, Hussein Abdullah, Barpete, Surendra, Akdogan, Guray, Aydin, Gulsum, Sancak, Cengiz, Ozcan, Sebahattin |
| Přispěvatelé: |
Uşak Üniversitesi |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
WOS: 000436522600048 Potato (Solanum tuberosum L.) is highly demandable world food crop and has become a staple food in many parts of the world including Turkey, India and China. Potato under in vitro culture condition is recalcitrant and genotype dependent. Therefore, we tested several factors to enhance regeneration and genetic transformation efficiency of Turkish potato genotypes. The influence of physical factors and conditions in Agrobacterium mediated transformation of the potato leaves was tested. Using the best combination (10 mg L-1 ZR, 10 mg L-1 NAA) with Agrobacterium-mediated transformation, we regenerated independent putative transformants 61.6% of the total explants cultured. GUS and nptII positive plants were confirmed by molecular analysis (PCR) that showed transformation rate of 24.16%. The protocol described here is simple, efficient, and produces transgenic plant in 7-8 weeks after inoculation with Agrobacterium bacterium. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) The present work is partially supported by the Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank Prof. Dr. Gungor Yilmaz (University of Gaziosmanpasa, Department of Field Crops) and Dr. Huseyin Onaran (Nigde Potato Research Institute) for providing the plant material. The authors would like to thank Dr Jitendra Kumar (Department of Plant Pathology, University of Minnesota, USA) for valuable suggestion and improvement of the present manuscript. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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