Study of linkage disequilibrium evolution in a wheat MAGIC population
| Autor: | Thepot, Stéphanie, Goldringer, Isabelle, Paux, Etienne, Mackay, Ian, Enjalbert, Jerome |
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| Přispěvatelé: | Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Bayer SAS, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Norwich Research Park, Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Symposium on evolutionary breeding in cereals Symposium on evolutionary breeding in cereals, Jan 2013, Birmingham, United Kingdom |
| Popis: | International audience; At an accelerating pace, advances in technology are providing an increasing number of molecular markers to the cereal geneticist. Most of these newly produced markers first need to be mapped before they can be used in breeding programmes. Although classical bi-parental segregating populations can be used for marker mapping, they are limited in value when mapping high numbers of markers, both because of a low rate of polymorphism and due to the limited probability of recombination between increasingly tightly linked markers. New populations based on multi-parental crosses and higher levels of recombination have been developed recently, for example MAGIC populations (Multi-parent Advanced Generation Inter-Cross), which overcome these drawbacks. The MAGIC wheat population we are studying is composed of 1100 SSD (Single Seed Descent) lines resulting from 12 cycles of random crosses of 62 founders with a large genetic base. These random crosses were facilitated by the integration of a nuclear male sterility allele (ms1b, Probus donnor) in the population. This population has been genotyped with an Illumina array of 9k SNPs (Single Nucleotide Polymorphisms), using a set of markers spread across the genome (Chao et al. 2010). The MAGIC parents were also genotyped with 3000 SNPs developed on chromosome 3B (Paux et al. 2006). Polymorphism and quality checks resulted in the selection of 6480 SNP markers from the 9k set, and 2690 SNP markers on the 3B chromosome. Before mapping markers, the genetic structure and linkage disequilibrium (LD) present in SSD lines and parental lines was assessed, confirming the efficiency of the 12 cycles of random crossing. Following this, the markers were mapped by means of a new method based on LD analysis, using the 3B dataset on the MAGIC parental panel. The quality of the method was assessed using previously mapped markers, or using 9k data on the SSD. The validity of the population for both marker mapping and quantitative trait locus (QTL) detection is discussed with regard to these first results. |
| Databáze: | OpenAIRE |
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