NON-SMOKY GLYCOSIDE GLYCOSYL TRANSFERASE prevents the release of smoky aroma from Tomato Fruit
| Autor: | Tikunov, Y.M, Molthoff, Jos, De vos, R.C.H, Beekwilder, J, Van Houwelingen, A, Van der Hooft, Justin J. J., Nijenhuis-de Vries, Mariska, Labrie, C.W, Verkerke, W, Van de Geest, H, Viquez Zamora, Marcela, Presa Castro, Silvia, Rambla Nebot, Jose Luis, Granell Richart, Antonio, Hall, R.D, Bovy, Arnaud G. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| Popis: | [EN] Phenylpropanoid volatiles are responsible for the key tomato fruit (Solanum lycopersicum) aroma attribute termed "smoky." Release of these volatiles from their glycosylated precursors, rather than their biosynthesis, is the major determinant of smoky aroma in cultivated tomato. Using a combinatorial omics approach, we identified the NON-SMOKY GLYCOSYLTRANSFERASE1 (NSGT1) gene. Expression of NSGT1 is induced during fruit ripening, and the encoded enzyme converts the cleavable diglycosides of the smoky-related phenylpropanoid volatiles into noncleavable triglycosides, thereby preventing their deglycosylation and release from tomato fruit upon tissue disruption. In an nsgt1/nsgt1 background, further glycosylation of phenylpropanoid volatile diglycosides does not occur, thereby enabling their cleavage and the release of corresponding volatiles. Using reverse genetics approaches, the NSGT1-mediated glycosylation was shown to be the molecular mechanism underlying the major quantitative trait locus for smoky aroma. Sensory trials with transgenic fruits, in which the inactive nsgt1 was complemented with the functional NSGT1, showed a significant and perceivable reduction in smoky aroma. NSGT1 may be used in a precision breeding strategy toward development of tomato fruits with distinct flavor phenotypes. We thank Syngenta Seeds, Seminis, Enza Zaden, Rijk Zwaan, Vilmorin, and de Ruiter Seeds for providing seeds of the 94 tomato cultivars. We acknowledge financial support from the Center for BioSystems Genomics, provided under the auspices of the Netherlands Genomics Initiative. R.C.H.d.V. and R.D.H. thank the Netherlands Metabolomics Centre for additional funding. We also thank the Metabolomics lab for assistance in volatile determination and Rafael Martinez at the Instituto de Biologia Molecular y Celular de Plantas for excellent plant management. We thank Gerco Angenent for discussions on the research and Ruud de Maagd for critical reading of the article. We also thank Fien Meijer-Dekens and A.W. van Heusden for excellent greenhouse management and plant cultivation. Finally, we thank Harry Jonker and Bert Schipper for preparation and analyses of the samples for LC-QTOF-MS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|