Morphological evidence for direct interaction between gonadotrophin-releasing hormone neurones and astroglial cells in the human hypothalamus.: Neuroglial interactions for GnRH neurones in the human hypothalamus
| Autor: | Baroncini, Marc, Allet, Cécile, Leroy, Daniel, Beauvillain, Jean-Claude, Francke, Jean-Paul, Prevot, Vincent |
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| Přispěvatelé: | Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Service de neurochirurgie, Hôpital Roger Salengro [Lille]-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
endocrine system
neuroendocrine brain MESH: Neurons MESH: Intermediate Filament Proteins LHRH tanycytes reproduction MESH: Aged 80 and over MESH: Gonadotropin-Releasing Hormone MESH: Cell Shape MESH: Glial Fibrillary Acidic Protein MESH: Animals human MESH: Nerve Tissue Proteins MESH: Neuronal Plasticity MESH: Aged MESH: Humans astrocytes MESH: Adult MESH: Hypothalamus MESH: Astrocytes nervous system MESH: Vimentin [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Neuroendocrinology Journal of Neuroendocrinology, Wiley, 2007, 19 (9), pp.691-702. ⟨10.1111/j.1365-2826.2007.01576.x⟩ Journal of Neuroendocrinology, 2007, 19 (9), pp.691-702. ⟨10.1111/j.1365-2826.2007.01576.x⟩ |
| ISSN: | 0953-8194 1365-2826 |
| DOI: | 10.1111/j.1365-2826.2007.01576.x⟩ |
| Popis: | International audience; In rodents, there is compelling evidence indicating that dynamic cell-to-cell communications involving cross talk between astroglial cells (such as astrocytes and specialised ependymoglial cells known as tanycytes) and neurones are important in regulating the secretion of gonadotrophin-releasing hormone (GnRH), the neurohormone that controls both sexual maturation and adult reproductive function. However, whether such astroglial cell-GnRH neurone interactions occur in the human brain is not known. In the present study, we used immunofluorescence to examine the anatomical relationship between GnRH neurones and glial cells within the hypothalamus of five women. Double-staining experiments demonstrated the ensheathment of GnRH neurone perikarya by glial fibrillary acidic protein (GFAP)-immunoreactive astrocyte processes in the periventricular zone of the tuberal region of the hypothalamus. GFAP immunoreactivity did not overlap that of GnRH at the GnRH neurone's projection site (i.e. the median eminence of the hypothalamus). Rather, human GnRH neuroendocrine fibres were found to be closely associated with vimentin or nestin-immunopositive radial glial processes likely belonging to tanycytes. In line with these light microscopy data, ultrastructural examination of GnRH-immunoreactive neurones showed numerous glial cells in direct apposition to pre-embedding-labelled GnRH cell bodies and/or dendrites in the infundibular nucleus, whereas postembedding immunogold-labelled GnRH nerve terminals were often seen to be enwrapped by glial cell processes in the median eminence. GnRH nerve button were sometimes visualised in close proximity to fenestrated pituitary portal blood capillaries and/or evaginations of the basal lamina that delineate the pericapillary space. In summary, these data demonstrate that GnRH neurones morphologically interact with astrocytes and tanycytes in the human brain and provide evidence that glial cells may contribute physiologically to the process by which the neuroendocrine brain controls the function of GnRH neurones in humans. |
| Databáze: | OpenAIRE |
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