TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis
| Autor: | Chen, Thomas T, Li, Li, Chung, Dong-Hui, Allen, Christopher DC, Torti, Suzy V, Torti, Frank M, Cyster, Jason G, Chen, Chih-Ying, Brodsky, Frances M, Niemi, Eréne C, Nakamura, Mary C, Seaman, William E, Daws, Michael R |
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| Rok vydání: | 2005 |
| Předmět: |
Kidney Disease
1.1 Normal biological development and functioning Green Fluorescent Proteins Immunology Renal and urogenital Fluorescent Antibody Technique Kidney Inbred C57BL Polymerase Chain Reaction Medical and Health Sciences Antibodies Cell Line Mice Underpinning research Monoclonal Animals 2.1 Biological and endogenous factors Aetiology DNA Primers B-Lymphocytes Tumor Liver Disease Membrane Proteins Molecular Flow Cytometry Immunohistochemistry Endocytosis Good Health and Well Being Liver Ferritins Digestive Diseases Cloning |
| Zdroj: | The Journal of experimental medicine, vol 202, iss 7 |
| Popis: | T cell immunoglobulin-domain and mucin-domain (TIM) proteins constitute a receptor family that was identified first on kidney and liver cells; recently it was also shown to be expressed on T cells. TIM-1 and -3 receptors denote different subsets of T cells and have distinct regulatory effects on T cell function. Ferritin is a spherical protein complex that is formed by 24 subunits of H- and L-ferritin. Ferritin stores iron atoms intracellularly, but it also circulates. H-ferritin, but not L-ferritin, shows saturable binding to subsets of human T and B cells, and its expression is increased in response to inflammation. We demonstrate that mouse TIM-2 is expressed on all splenic B cells, with increased levels on germinal center B cells. TIM-2 also is expressed in the liver, especially in bile duct epithelial cells, and in renal tubule cells. We further demonstrate that TIM-2 is a receptor for H-ferritin, but not for L-ferritin, and expression of TIM-2 permits the cellular uptake of H-ferritin into endosomes. This is the first identification of a receptor for ferritin and reveals a new role for TIM-2. |
| Databáze: | OpenAIRE |
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