Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays

Autor: Raoux, Matthieu, Bornat, Yannick, Quotb, Adam, Catargi, Bogdan, Renaud, Sylvie, Lang, Jochen
Přispěvatelé: Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), CHU Bordeaux [Bordeaux], Renaud, Sylvie
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: The Journal of Physiology
The Journal of Physiology, Wiley, 2011, 590 (5), pp.1085-1091
ISSN: 0022-3751
1469-7793
Popis: International audience; Non-invasive high-throughput and long-term monitoring of endocrine cells is important for drug research, phenotyping, tissue engineering and pre-transplantation quality control. Here we report a novel approach to obtain simultaneous long-term electrical recordings of different islet cell types using multi-electrode arrays. We implemented wavelet transforms to resolve the low signal/noise ratio inherent to these measurements and extracted on-line a signature specific of cell activity. The architecture employed allows multiplexing a large number of electrodes for high-throughput screening. This method should be of considerable advantage in endocrine research and may be extended to other excitable cells previously not accessible to the technique.
Databáze: OpenAIRE
Externí odkaz: