| Autor: |
Ball, G, Demmerle, J, Kaufmann, R, Davis, I, Dobbie, IM, Schermelleh, L |
| Rok vydání: |
2016 |
| Předmět: |
|
| DOI: |
10.1038/srep15915 |
| Popis: |
Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms and will promote routine application of super-resolution SIM imaging in cell biology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
