Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells
| Autor: | Dane-Oorschot, A.A.A.M. van, Fischer, D.F., Grimbergen, J.M., Klein, B., Zhuang, S.M., Falkenburg, J.H.F., Backendorf, C., Quax, P.H.A., Eb, A.J. van der, Noteborn, M.H.M. |
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| Přispěvatelé: | Gaubius Instituut TNO |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Male
Skin Physiology Circoviridae T-Lymphocytes Apoptosis Gallus gallus Simian virus 40 Fibroblasts Transfection Recombinant Proteins Capsid Cell Transformation Neoplastic Tumor Cells Cultured Humans Capsid Proteins Fluorescent Antibody Technique Indirect Biology Cells Cultured Chicken anemia virus Cell Line Transformed Skin |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, 11, 94, 5843-5847 |
| Popis: | The chicken anemia virus protein apoptin induces a p53-independent, Bcl- 2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro, apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become susceptible to apoptosis by apoptin. Long-term expression of apoptin in normal human fibroblasts revealed that apoptin has no toxic or transforming activity in these cells. In normal cells, apoptin was found predominantly in the cytoplasm, whereas in transformed and malignant cells it was located in the nucleus, suggesting that the localization of apoptin is related to its activity. These properties make apoptin a potential agent for the treatment of a large number of tumors, also those lacking p53 and/or overexpressing Bcl-2. |
| Databáze: | OpenAIRE |
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