Androgen Regulation of micro-RNAs in Prostate Cancer
| Autor: | Waltering, KK, Porkka, KP, Jalava, SE, Urbanucci, A, Kohonen, PJ, Latonen, LM, Kallioniemi, OP, Jenster, Guido, Visakorpi, T |
|---|---|
| Přispěvatelé: | Urology |
| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Waltering, K K, Porkka, K P, Jalava, S E, Urbanucci, A, Kohonen, P J, Latonen, L M, Kallioniemi, O P, Jenster, G & Visakorpi, T 2011, ' Androgen regulation of micro-RNAs in prostate cancer ', The Prostate, vol. 71, no. 6, pp. 604-614 . https://doi.org/10.1002/pros.21276 Prostate, 71(6), 604-614. Wiley-Liss Inc. |
| ISSN: | 0270-4137 |
| DOI: | 10.1002/pros.21276 |
| Popis: | BACKGROUND. Androgens play a critical role in the growth of both androgen dependent and castration-resistant prostate cancer (CRPC). Only a few micro-RNAs (miRNAs) have been suggested to be androgen regulated. We aim to identify androgen regulated miRNAs. METHODS. We utilized LNCaP derived model, we have established, and which over-expresses the androgen receptor (AR), the VCaP cell line, and 13 intact-castrated prostate cancer (PC) xenograft pairs, as well as clinical specimens of untreated (PC) and CRPC. The expression of miRNAs was analyzed by microarrays and quantitative RT-PCR (Q-RT-PCR). Transfection of pre-miR-141 and anti-miR-141 was also used. RESULTS. Seventeen miRNAs were > 1.5-fold up-or downregulated upon dihydrotestosterone (DHT) treatment in the cell lines, and 42 after castration in the AR-positive xenografts. Only four miRNAs (miR-10a, miR-141, miR-150*, and miR-1225-5p) showed similar androgen regulation in both cell lines and xenografts. Of those, miR-141 was found to be expressed more in PC and CRPC compared to benign prostate hyperplasia. Additionally, the overexpression of miR-141 enhanced growth of parental LNCaP cells while inhibition of miR-141 by anti-miR-141 suppressed the growth of the LNCaP subline overexpressing AR. CONCLUSIONS. Only a few miRNAs were found to be androgen-regulated in both cell lines and xenografts models. Of those, the expression of miR-141 was upregulated in cancer. The ectopic overexpression of miR-141 increased growth of LNCaP cell suggesting it may contribute to the progression of PC. Prostate 71: 604-614, 2011. (C) 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|