| Autor: |
Mironov V., Shirshikov F., Kalacheva N., Cherepnev G. |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
SCOPUS1815445X-2012-7-3-SID84871834497 |
| Popis: |
Effect of binase (RNAse of Bacillus intermedius) on phorbol myristate acetate-(PMA)-induced apoptosis of human peripheral blood granulocytes and monocytes was studied in vitro by flow cytometry. Both toxic (400 μg/ml) and nontoxic (40 μg/ml) binase concentrations were tested. The binase end-point effect was dependent on the target cell population and the binase concentration. In a granulocyte subset, the 400 μg/ml concentration resulted in strongly pronounced stimulation of PMA-induced apoptosis. In a monocyte subset, the 40 μg/ml concentration developed a protective effect as judged by an increase in a percantage of viable cell subset and by slowing-down cells transtion from an early to late PMA-induced apoptotic phase. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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