Comparative Evaluation of Protease Extraction from Leaves of Syzygium polyanthum

Autor:	Yahya, Noorhafiza, Mohd Arshad, Zatul Iffah, Mudalip, Siti Kholijah Abdul, Sulaiman, Siti Zubaidah, Man, Rohaida Che, Md Shaarani, Shalyda, Ab. Rahim, Mohd Hairul
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Syzigium polyanthum Chemistry plant protease protease extraction QD1-999
Zdroj:	Indonesian Journal of Chemistry, Vol 19, Iss 2, Pp 503-510 (2019) Indonesian Journal of Chemistry; Vol 19, No 2 (2019); 503-510
ISSN:	2460-1578 1411-9420
Popis:	In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogen-bond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::4d5efd1feb80d78a4ddd87d92c525357 https://jurnal.ugm.ac.id/ijc/article/view/40860 Zobrazit plný text záznamu