Memórias do Instituto Oswaldo Cruz
| Autor: | Atta, AM, Sousa-Atta, MLB, D'Oliveira, A, Almeida, RP, Araújo, MI, Carvalho, EM |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA Memórias do Instituto Oswaldo Cruz, Volume: 97, Issue: 1, Pages: 101-103, Published: JAN 2002 |
| DOI: | 10.1590/S0074-02762002000100017 |
| Popis: | p. 101-103 Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-17T15:34:45Z No. of bitstreams: 1 4291.pdf: 74717 bytes, checksum: 58e675ae0f403132ba5858e62a9b23c7 (MD5) Made available in DSpace on 2012-12-17T15:34:45Z (GMT). No. of bitstreams: 1 4291.pdf: 74717 bytes, checksum: 58e675ae0f403132ba5858e62a9b23c7 (MD5) Previous issue date: 2002-01 Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera. |
| Databáze: | OpenAIRE |
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