Gut microbiome compositional differences between tumor and Non-tumor adjacent tissues from US and Spain identifies eikenella as a potential new player in colorectal cancer

Autor: Allali, I, Delgado, Susana, Astudillo, A, Ghazal, Hassan, Amzazi, Saaïd, Yeh, Jen Jen, Keku, Temitope, Azcárate-Peril, M. Andrea
Rok vydání: 2015
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Trabajo presentado en el 5th International Human Microbiome Congress, IHMC, celebrado en Luxemburgo del 31 de marzo al 2 de abril de 2015
Colorectal cancer (CRC) is the third most common cancer in the world and the second leading cause of cancer deaths in the US and Spain. The molecular mechanisms involved in the etiology of CRC are not yet elucidated due in part to the complexity of the human gut microbiota. In this study, we compared the microbiome composition of 102 tumor and matching adjacent normal tissue (normal) from cohorts from the US and Spain by 16S rRNA amplicon sequencing in order to determine the impact of the geographic origin on the CRC microbiome. Data showed a significantly (P < 0.05) higher Phylogenetic Diversity (PD) for the US (PD Normal = 26.3 ± 5.3, PD Tumor = 23.3 ± 6.2) compared to the Spanish cohort (PD Normal = 18.9 ± 5.9, PD Tumor = 18.7 ± 6.6) while no significant differences in bacterial diversity were observed between tumor and normal tissues for individuals from the same country. Normal tissues from the Spanish cohort were enriched in Firmicutes (43.9% and 22.2%, P = 0.0001) and Actinobacteria (1.6% and 0.5%, P = 0.0018) compared to US normal tissues, while normal tissues from the US had significantly higher abundances of Fusobacteria (8.1% and 1.5%, P = 0.0023) and Sinergistetes (0.3% and 0.1%, P = 0.0097). Comparison of tumor and normal tissues in each cohort identified the genus Eikenella significantly over represented in US tumors (0.024% and 0%, P = 0.03), and the genera Fusobacterium (10.4% and 1.5%, P = < 0.0001), Bulleida (0.36% and 0.09%, P = 0.02), Gemella (1.46% and 0.19%, P = 0.03), Parvimonas (3.14% and 0.86%, P = 0.03), Campylobacter (0.15% and 0.008%, P = 0.047), and Streptococcus (2.84% and 2.19%, P = 0.05) significantly over represented in Spanish tumors. Our study suggests that microbiome compositional dissimilarities by geographic location should be taken in consideration when approaching CRC therapeutic options.
Databáze: OpenAIRE
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