Cerebral MRI and MRS in newborn piglets with and without seizures in a lipopolysaccharide-sensitized hypoxic cardiac arrest model
| Autor: | Kasper Seiersen, Hannah Brogård Andersen, Mads Andersen, Ted Carl Kejlberg Andelius, Mette Vestergård Pedersen, Lærke Hjøllund Hansen, Bo Løfgren, Steffen Ringgaard, Kasper Jacobsen Kyng, Tine Brink Henriksen |
|---|---|
| Zdroj: | Aarhus University Seiersen, K V, Brogård Andersen, H, Andersen, M, Andelius, T C K, Pedersen, M V, Hansen, L H, Løfgren, B, Ringgaard, S, Kyng, K J & Henriksen, T B 2022, ' Cerebral MRI and MRS in newborn piglets with and without seizures in a lipopolysaccharide-sensitized hypoxic cardiac arrest model ', Frontiers in pediatrics . |
| Popis: | Background and AimsSeizures in relation to neonatal hypoxic-ischemic brain injury have been associated with poor neurological outcome. However, the potential underlying pathology is poorly understood. We aim to study the impact of post cardiac arrest (CA) seizures on magnetic resonance imaging (MRI) and spectroscopy (MRS) outcomes in a newborn piglet model of lipopolysaccharide (LPS) sensitized CA.MethodsIn newborn piglets, systemic inflammation was induced by infusion of the bacterial endotoxin LPS under general anesthesia. After four hours of LPS infusion, CA was induced by endotracheal tube clamping. The piglets were randomized to either resuscitation with epinephrine or placebo. Seizures were detected clinically and/or by amplitude integrated electroencephalography during the observational period. Several MRI and MRS parameters were assessed after 14 hours, including perfusion evaluated by arterial spin labelling.Results30 piglets were included and randomized, of which 1 died during resuscitation, and 7 died prior to MRI and MRS. No difference in seizure frequency by epinephrine (+/-) was detected (6/14 piglets resuscitated with epinephrine: 7/15 piglets with placebo). When compared to piglets without seizure, piglets with seizures had increased cerebral perfusion; epinephrine: 162 (77) vs 44 (9) ml/min/100g, p = 0.02, Placebo: 192 (78) vs 51 (6) ml/min/100 g, p = 0.02. We found no statistically significant differences in any of the other MRI/MRS outcomes.ConclusionsAt 14 hours after CA, perfusion was increased in piglets with post-CA seizures. Seizures may result in increased perfusion due to altered cerebral metabolism, which in turn may contribute to poor neurological outcome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|