Influence of a hypercholesterolemic diet on the collagen composition of the bladder wall extracellular matrix in rats
| Autor: | Nunes, R. L. V., Bruschini, H., Utsunomia, K., Silveira, M. A., Walcy Rosolia Teodoro, Leite, K. R. M., Srougi, M. |
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| Zdroj: | Scopus-Elsevier DIGITUM. Depósito Digital Institucional de la Universidad de Murcia instname ResearcherID |
| Popis: | Purpose: To investigate the effects of hypercholesterolemic diet on the collagen composition of urinary bladder wall. Materials and methods: Forty-five female 4-week-old Wistar rats were divided into three groups: 1) control group fed a normal diet (ND); 2) model of bladder outlet obstruction (BOO) group fed a ND; and 3) group fed a HCD (1.25% cholesterol). Total serum cholesterol, LDL cholesterol and body weight were assessed at baseline. Four weeks later, group 2 underwent a surgical procedure resulting in a partial BOO, while groups 1 and 3 underwent a sham similar surgical procedure. Six weeks later, all animals had their bladders removed; serum cholesterol and LDL cholesterol levels and body weights were measured. Morphological and morphometric analysis was performed by Picrosirius staining and collagen types I and III were identified by immunofluorescence. Statistical analysis was completed and significance was considered when p |
| Databáze: | OpenAIRE |
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