Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning
| Autor: | Mayoral I, Bevilacqua E, Gómez G, Hmadcha A, González-Loscertales I, Reina E, Sotelo J, Domínguez A, Pérez-Alcántara P, Smani Y, González-Puertas P, Mendez A, Uribe S, Smani T, Ordoñez A, Valverde I |
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| Rok vydání: | 2022 |
| Předmět: |
mesenchymal stem cells
MSC extracellular matrix ECM vascular smooth muscle cells VSMC anti-fibroblast specific protein 1 FSP1 wall shear stress WSS Vascular graft Endothelin Receptor B ETB transforming growth factor beta 1 TGFß-1 tissue engineering vascular grafts TEVG Three-dimensional 3D Reverse Transcription Rt Tissue engineering derived VSMC dVSMC anti-cluster of differentiation 31 CD31 Electrospinning Endothelin Receptor A ETA platelet-derived growth factor composed by two beta chains PDGF-BB 3D printing computed tomography CT bone morphogenetic protein BMP4 computation fluid dynamic CFD western blotting WB anti-smooth muscle protein 22 SM-22 fused deposition modelling FDM Mesenchymal stem cells room temperature RT anti-alpha-smooth muscle actin a-SMA endothelin-1 ET-1 |
| Zdroj: | Materials Today Bio r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante instname |
| ISSN: | 2590-0064 |
| Popis: | Three-dimensional (3D) engineered cardiovascular tissues have shown great promise to replace damaged structures. Specifically, tissue engineering vascular grafts (TEVG) have the potential to replace biological and synthetic grafts. We aimed to design an in-vitro patient-specific patch based on a hybrid 3D print combined with vascular smooth muscle cells (VSMC) differentiation. Based on the medical images of a 2 months-old girl with aortic arch hypoplasia and using computational modelling, we evaluated the most hemodynamically efficient aortic patch surgical repair. Using the designed 3D patch geometry, the scaffold was printed using a hybrid fused deposition modelling (FDM) and electrospinning techniques. The scaffold was seeded with multipotent mesenchymal stem cells (MSC) for later maturation to derived VSMC (dVSMC). The graft showed adequate resistance to physiological aortic pressure (burst pressure 101 ?± ?15 ?mmHg) and a porosity gradient ranging from 80 to 10 ?µm allowing cells to infiltrate through the entire thickness of the patch. The bio-scaffolds showed good cell viability at days 4 and 12 and adequate functional vasoactive response to endothelin-1. In summary, we have shown that our method of generating patient-specific patch shows adequate hemodynamic profile, mechanical properties, dVSMC infiltration, viability and functionality. This innovative 3D biotechnology has the potential for broad application in regenerative medicine and potentially in heart disease prevention. |
| Databáze: | OpenAIRE |
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