Antinociceptive properties and acute toxicity of ethanol extract of Bromelia laciniosa Mart. ex Schult. f. (Bromeliaceae)
| Autor: | de Lima-Saraiva, SRG, Saraiva, HCC, Silva, JC, Neves, LF, Damasceno, PKF, Branco, CRC, Branco, A, Amorim, ELC, Almeida, JRGS |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 13, No 10 (2014); 1659-1666 |
| ISSN: | 1596-5996 1596-9827 1659-1666 |
| Popis: | Purpose: To investigate the antinociceptive activity and acute toxicity of the ethanol extract of Bromelia laciniosa leaf.Methods: A high performance liquid chromatography HPLC fingerprint of phenolic compounds was developed. The antinociceptive effect of ethanol extract (Bl-EtOH) in mice was carried out using chemical (writhing and formalin) and thermal (hot plate) models of nociception. The acute toxicity of the extract was performed in mice using doses of 2.0 g/kg intraperitoneally and 5.0 g/kg orally. Blood was removed for laboratory analysis of hematological and biochemical parameters.Results: Bl-EtOH (100, 200 and 400 mg/kg, i.p.) reduced the number of writhing (91.80, 93.44 and 78.68 %, respectively) and the number of paw licks during the first (60.86, 62.84 and 66.79 %) and second phase (91.93, 82.18 and 88.73 %) of the formalin test. Naloxone (1.5 mg/kg, i.p.) antagonized the antinociceptive action of Bl-EtOH (100 mg/kg), and this finding suggests involvement of opioid mechanism. The effect of Bl-EtOH on hot plate response provides a confirmation of its central effect.Conclusion: B. laciniosa leaf extract has antinociceptive properties. Peripheral, and at least in part, central mechanisms, may be involved in this antinociceptive effect. The ethanol leaf extract apparently presents no significant toxicity.Keywords: Bromelia laciniosa, Nociception, Pain, Writhing, Acute toxicity |
| Databáze: | OpenAIRE |
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