| Autor: |
Štefanac, Ivan, Mrčela, Milanka, Trnski, Diana, Sabol, Maja, Musani, Vesna, Ozretić, Petar, Levanat, Sonja |
| Přispěvatelé: |
Massi, Daniela |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.1007/s00428-019-02631-8 |
| Popis: |
Background & Objectives: The Hedgehog-GLI (HH- GLI) signalling pathway is primarily associated with embryonic development but its role in carcinogenesis has being intensely studied in the last two decades. Recent research indicates that HH-GLI pathway could be a key player in prostate cancer (PC) development and progression, as well as therapeutic resistance. The main objective of this study was to investigate the HH-GLI pathway activity in PC in comparison with healthy prostate and prostate inflammation. Methods: Around 30 formalin-fixed paraffin-embedded tissue samples per group were collected from PC patients (Grade Groups I-V) and two controls groups (benign prostate tissue and prostate inflammation). Expression profiles of GLI3 and PTCH1 proteins was detemined immunohistochemically. The level of protein staining was expressed by multiplying percentage of positive stained cells and staining intensity (histoscore), separately for prostate epithelium and stroma. Cellular localization of protein staining (nuclear and/or cytoplasmic) was also determined. Results: GLI3 and PTCH1 were overexpressed in epithelium (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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