| Autor: |
De Boer, Monica L., Kum, Winnie W.S., Pang, Liwina T.Y., Chow, Anthony W. |
| Zdroj: |
Microbial Pathogenesis; August 1999, Vol. 27 Issue: 2 p61-70, 10p |
| Abstrakt: |
It has previously been reported that staphylococcal enterotoxin A (SEA) is frequently co-expressed with toxic shock syndrome toxin-1 (TSST-1) in menstrual Toxic Shock Syndrome (MTSS)-associated Staphylococcus aureus. It was hypothesized that co-production of SEA and TSST-1 might yield a more virulent strain than one that produced TSST-1 but not SEA. To test this hypothesis, a TSST-1+/SEA−derivative of S. aureusRN3984 (TSST-1+/SEA+) was constructed by plasmid integration, and the isogenic pair were introduced into a D-galactosamine sensitized mouse model of lethal shock. At 72 h, 27 out of 30 (90%) mice inoculated with the parental strain died, as compared to 21 out of 30 (70%) mice inoculated with the isogenic derivative (P=0.05, Fisher's exact test; 1-tailed; 95% confidence limits, 0.80–20.80). Our results suggest that co-production of SEA with TSST-1 does enhance the ability of this strain of S. aureusto induce lethal shock in vivo. This enhanced virulence could be due to an additive or synergistic activity of the toxin combination on T cell proliferation and cytokine production in the animal model. |
| Databáze: |
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