| Autor: |
Wojtczak, Lech, Nikitina, Elena R., Czyż, Aneta, Skulskii, Igor A. |
| Zdroj: |
Biochemical and Biophysical Research Communications; June 1996, Vol. 223 Issue: 2 p468-473, 6p |
| Abstrakt: |
Cuprous ions at micromolar concentrations induced swelling of rat liver mitochondria in isotonic solutions of potassium thiocyanate and potassium acetate. The swelling in K-acetate in the presence of the protonophere carbonyl cyanidem-chloropenylhydrazone was partly inhibited by glibenclamide. In K+-containing media, Cu+collapsed the mitochondrial membrane potential formed by operation of the respiratory chain with succinate or tetramethylp-phenylenediamine + ascorbate as substrates or by the proton-pumping ATPase. In contrast, in K+-free media, isotonic sucrose or choline chloride, but not in NaCl, Cu+induced a transient potassium gradient potential. These results indicate that cuprous ions at low concentrations, apart from promoting the electroneutral K+/H+exchange, facilitate the uniport of K+, presumably by activating the mitochondrial potassium channel sensitive to glibenclamide. |
| Databáze: |
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