Comparison of emmTyping and Ribotyping with Three Restriction Enzymes To Characterize Clinical Isolates of Streptococcus pyogenes

Autor: Doktor, Stella Z., Beyer, Jill M., Flamm, Robert K., Shortridge, Virginia D.
Zdroj: Journal of Clinical Microbiology; January 2005, Vol. 43 Issue: 1 p150-155, 6p
Abstrakt: ABSTRACTA total of 336 Streptococcus pyogenesisolates recently recovered from patients with pharyngitis from 13 countries were characterized by emmtyping and riboprinting using an automated Riboprinter (Dupont/Qualicon) based on the patterns produced by three restriction enzymes, EcoRI, PstI, and HindIII. Three enzymes were necessary to increase the discrimination of ribogroups formed by each enzyme. A total of 40 ribogroups and 38 emmsequences (not counting allelic variations) were identified. Multilocus sequence typing was performed on a sampling of the isolates, and those results were consistent with those of both emmtyping and ribotyping. Correlations were observed among all three methods.
Databáze: Supplemental Index