| Autor: |
Xu, M., Geer, L. Y., Bryant, S. H., Roth, J. S., Kowalak, J. A., Maynard, D. M., Markey, S. P. |
| Zdroj: |
Journal of Proteome Research; April 2005, Vol. 4 Issue: 2 p300-305, 6p |
| Abstrakt: |
An algorithm is introduced to assess spectral quality for peptide CID spectra acquired by a quadrupole ion trap mass spectrometer. The method employs a quadratic discriminant function calibrated with manually classified bad' and good' quality spectra, producing a single spectral quality' score. Many spectra examined that do not have significant matches are assessed to have good spectral quality, indicating that advances in search methods may yield substantial improvements in results. Keywords: shotgun proteomics • mass spectral quality • peptide sequence • CID • quadrupole ion trap mass spectrometry |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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