| Autor: |
McClenaghan, Neville H., Gray, Alison M., Barnett, Christopher R., Flatt, Peter R. |
| Zdroj: |
Biochemical and Biophysical Research Communications; June 1996, Vol. 223 Issue: 3 p724-728, 5p |
| Abstrakt: |
Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake of D-glucose, significantly greater than RINm5F after only 5 min (p<0.05). Whereas BRIN-BD11 cells expressed a high glucokinase:hexokinase ratio with 1.4–2.0 fold and 1.4–1.7 fold stepwise stimulation of insulin secretion with 4.2–16.7 mM D-glucose and D-mannose respectively, RINm5F had a lower glucokinase:hexokinase ratio (p<0.001) and were notably unresponsive to D-glucose and D-mannose. Unlike RINm5F cells, BRIN-BD11 were unresponsive to other hexoses, with RINm5F only responding to D-galactose (p<0.05). BRIN-BD11 cells should be useful for studies of nutrient-induced insulin secretion. |
| Databáze: |
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