| Autor: |
Bömer, Ulf, Rassow, Joachim, Zufall, Nicole, Pfanner, Nikolaus, Meijer, Michiel, Maarse, Ammy C. |
| Zdroj: |
JMB Online (Journal of Molecular Biology); October 4, 1996, Vol. 262 Issue: 4 p389-395, 7p |
| Abstrakt: |
The preprotein translocase of the inner mitochondrial membrane has only been described inSaccharomyces cerevisiaeto date. We report that the essential subunit Tim17 is highly conserved in evolution. The targeting and assembly of yeast Tim17 as well as that of human andDrosophila melanogasterTim17 were characterized with isolated yeast mitochondria. Targeting signals in the mature protein direct the Tim17 precursors to the receptor Tom70 on the mitochondrial surface. In a membrane potential-dependent step the precursors insert into the inner membrane, adopt a characteristic topology and assemble with Tim23. The mechanisms of targeting and assembly were indistinguishable between the Tim17s from distinct organisms, indicating a high evolutionary conservation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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