| Autor: |
Gregorc, Aleš, Pogacnik, Azra, Bowen, Ivor D. |
| Zdroj: |
Apidologie; 2004, Vol. 35 Issue: 5 p453-460, 8p |
| Abstrakt: |
The effects of oxalic (OA) and formic acids (FA) on honeybee larvae in colonies were assessed and evaluated. Cell death was detected by the TUNEL technique for DNA labelling. In 3- and 5-day-old larvae exposed to OA, cell death was found in 25% of midgut epithelial cells 5 h after the treatment, using an In situ cell death detection kit, AP (Roche). The level of cell death increased to 70% by the 21st hour and the morphology of the epithelium remained unchanged. Fifty hours after the application, cell death was established in 18% of the epithelial cells of the 3-day-old larvae and had increased to 82% in the 5-day-old larvae. A DeadEnd apoptosis detection kit (Promega) showed sporadic cell death mainly in the larval fat body 5 h after treatment. Twenty-one hours after the OA application cell death was found in 4% of the larval midgut epithelial cells. Evaporated formic acid induced extensive apoptotic cell death in the peripheral, cuticular and subcuticular tissues that preceded the cell death of the entire larval body. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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