| Autor: |
Park, S., Pastuszak, I., Mengeling, B.J., Turco, S.J., Elbein, A.D. |
| Zdroj: |
Analytical Biochemistry; January 1997, Vol. 244 Issue: 2 p321-327, 7p |
| Abstrakt: |
We describe a procedure for the enzymatic synthesis of labeled or unlabeled GDP-d-arabinopyranoside. This method uses two enzymes purified from pig kidney: anl-fucokinase and a GDP-l-fucose pyrophosphorylase. The isolated GDP-d-[3H]arabinose served as a precursor for arabinose addition to lipophosphoglycan (LPG) ofLeishmania major,using a parasite membrane fraction as the source of arabinosyltransferase. The procedures described provide a useful means for obtaining radiolabeled GDP-d-arabinopyranoside to study synthesis ofd-arabinopyranoside-containing glycoconjugates. |
| Databáze: |
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