| Autor: |
Zhang, Enshuo, Zhao, Baicheng, Liu, Yupeng, Liu, Gang, Lu, Shuting, Xiong, Liqin, Pan, Xufeng, Yang, Haitang, Yao, Feng |
| Zdroj: |
STAR Protocols; 20240101, Issue: Preprints |
| Abstrakt: |
Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring post-operative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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