| Autor: |
Wang, Long, Hu, Ruizhi, Ma, Siqi, Yang, Xizi, Gong, Jiatai, Xiang, Hongkun, Shi, Mingkun, Yuan, Xupeng, Chen, Liang, Zhang, Hongfu, Tan, Bie, He, Xi, He, Jianhua, Wu, Shusong |
| Zdroj: |
Animal Nutrition; 20240101, Issue: Preprints |
| Abstrakt: |
Gut microbiota disruption during the weaning process is a significant factor of intestinal injury. Our previous studies have suggested that Prevotellamay play a critical role in causing intestinal inflammation. This study aimed to clarify the impact of Prevotella coprion intestinal injury and the protecting effect by dihydroquercetin (DHQ) in weaned piglets. A total of 108 healthy Duroc × Landrace × Yorkshire weaned piglets, aged 21 d, were randomly allocated into 3 groups with 6 replicates and 6 piglets per replicate. The piglets were the following diets for 28 d: 1) a basal diet, 2) basal diet containing 1.0 × 108CFU/kg P. copri, 3) basal diet supplemented with 1.0 × 108CFU/kg P. copriand 100 mg/kg DHQ. Results showed that P.copri decreased significantly the average daily gain (ADG) (P< 0.001), which was recovered by supplementation of DHQ with decreased serum levels of malondialdehyde (MDA), interleukin (IL)-2 and IL-8 but increased total superoxide dismutase (T-SOD) activity and IL-10 in weaned piglets (P< 0.001). Moreover, DHQ increased the expression of tight junction proteins (claudin-2, occludin and tight junction protein zonula occludens protein-1 (ZO-1) and the mRNA expression of glutathione peroxidase 4 (GPX-4) in ileum (P< 0.001). Intestinal flora analysis showed that P. copriincreased the relative abundance of Prevotella(P =0.026) and Eubacterium coprostanoligenes group(P< 0.001), but decreased the relative abundance of Lachnospiraceae NK4A136 group(P< 0.001), while supplementation of DHQ reduced the relative abundance of Prevotella(P =0.026). Metabolomics results indicated that P. coprienhanced the content of 12-OH bile acid, but decreased the contents of glycodeoxycholic acid (GDCA) and glycochenodeoxycholic acid (GCDCA) (P< 0.001), while DHQ reduced the 12-OH bile acid content (P< 0.001) and increased the GDCA content (P =0.020). In summary, P. copricaused intestinal injury and reduced growth performance in weaned piglets, and DHQ showed a protective effect by modulating gut microbiota and bile acids metabolism. |
| Databáze: |
Supplemental Index |
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