Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.

Autor: Satchwell, S. C., Travers, A. A.
Zdroj: The EMBO Journal; January 1989, Vol. 8 Issue: 1 p229-238, 10p
Abstrakt: Nucleosome dimers containing, on average, a single molecule of histone H5 have been isolated from chicken erythrocyte nuclei and the associated DNA fragments cloned and sequenced. The average sequence organization of at least one of the two nucleosomes in the dimers is highly asymmetric and suggests that the torsional, as well as the axial, flexibility of DNA is a determinant of nucleosome positioning. On average the nucleosome dimer is a polar structure containing linker DNA of variable lengths. The sequences associated with H5 containing nucleosomes and core particles are sufficiently different to indicate that removal of histone H5 (or H1) from chromatin may result in the migration of the histone octamer and a consequent exposure of sites for regulatory proteins.
Databáze: Supplemental Index