| Autor: |
Paucha, E., Kalderon, D., Richardson, W.D., Harvey, R.W., Smith, A.E. |
| Zdroj: |
The EMBO Journal; December 1985, Vol. 4 Issue: 12 p3235-3240, 6p |
| Abstrakt: |
We have examined the large T encoded by an SV40 mutant, d10, which fails to localize to the nucleus. The DNA sequence of the mutant predicts the alteration of Lys 128‐‐‐‐Thr within the sequence 127 Lys Lys Lys Arg Lys 131 of large T. The results show that d10 large T is capable of binding to SV40 DNA, to cellular DNA and to the cellular phosphoprotein p53 as well as wild‐type large T. These data suggest that the cytoplasmic location of d10 large T is not due to an inability of the protein to be retained within the nucleus, but argues instead that the protein fails to reach the nucleus because it contains a defective nuclear location signal. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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