Characterization of Bipolaris bicolorgermination: effects of a physical factor on fungal adaptability

Autor: Muniz, Paulo Henrique Pereira Costa, de Oliveira, Thiago Alves Santos, Duarte, Elizabeth Amélia Alves, Rodrigues, Fabrício, Carvalho, Daniel Diego Costa
Zdroj: Brazilian Journal of Microbiology; 20240101, Issue: Preprints p1-8, 8p
Abstrakt: Studies on physiological responses to stimuli from physical factors are essential for understanding the dynamics of the microorganisms and higly important for the management of plant diseases. Besides, the development of an epidemiological model for pathogen populations requires studying their physiological responses to physical stimuli. The objective of this study was to evaluate the germination dynamics of spores from six isolates of Bipolaris bicolorunder effects of light at 25 °C. Suspensions of 1.6 × 105conidia mL− 1from the B. bicolorisolates were inoculated onto Petri dishes containing agar-water culture medium and incubated in a BOD chamber under two physical conditions: (a) constant darkness and (b) constant light for five hours. The study was conducted in a completely randomized design, with a 6 × 2 factorial arrangement (six B. bicolorisolates and two physical conditions) and five replications. The length of the germ tube was measured hourly. The constant darkness resulted in higher mean germ tube growth for the pathogen; however, differences in the final germination percentage were found among the isolates. The isolate F-24-02 exhibited the highest germination adaptability to constant darkness, presenting the longest germ tube length.
Databáze: Supplemental Index