| Autor: |
Houston, Simon, Gomez, Alloysius, Geppert, Andrew, Goodyear, Mara C., Cameron, Caroline E. |
| Zdroj: |
Journal of Proteome Research; May 2024, Vol. 23 Issue: 5 p1725-1743, 19p |
| Abstrakt: |
Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all Treponema pallidumproteins under infection conditions (in vivo-grown T. pallidum). Recently, a method was developed for the long-term culture of T. pallidumunder in vitro conditions (in vitro-cultured T. pallidum). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the T. pallidumglobal protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined T. pallidumproteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of T. pallidum. Further, comparison of the in vitro-expressed proteome with the previously determined in vivo-expressed proteome identifies only a few proteomic changes between the two growth conditions, reinforcing the suitability of in vitro-cultured T. pallidumas an alternative to rabbit-based treponemal growth. The MS proteomics data have been deposited in the MassIVE repository with the data set identifier MSV000093603 (ProteomeXchange identifier PXD047625). |
| Databáze: |
Supplemental Index |
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