| Abstrakt: |
Objectives The objective of this research was to investigate the potential anti-inflammatory properties of the Cucurbita pepofruit. Given the adverse effects associated with long-term use or excessive doses of conventional anti-inflammatory medications, exploring herbal therapies as alternatives has become increasingly important.Methods Various preliminary tests and antioxidant assays, both enzymatic and non-enzymatic, were conducted on the C. pepofruit. Bioactive substances present in the fruit, including alkaloids, carbohydrates, flavonoids, tannins, amino acids, triterpenoids, and saponin glycosides, were identified through early analysis. Different extraction solvents, such as chloroform, methanol, and water, were utilized to extract samples. The methanolic extract was subjected to further testing for its in vitroanti-inflammatory characteristics. This involved evaluating their ability to stabilize RBC membranes, inhibit protein denaturation, and suppress proteinase activity using two concentrations (150 µg/ml and 300 µg/ml) of the extract.Results The methanolic extract of the C. pepofruit exhibited strong free radical scavenging activity in both DPPH and H2O2assays. Moreover, it demonstrated the dose-dependent stabilization of RBC membranes, resulting in reduced hemolysis, protein denaturation, and proteinase activity. These findings suggest potent anti-inflammatory effects of the methanolic extract.Conclusion This research study has found that the C. pepofruit methanolic extract significantly reduced inflammation. This demonstrates the promise of natural chemicals as less risky substitutes for traditional anti-inflammatory medications. Potentially safe and efficient treatments for inflammatory illnesses could be found in herbal remedies such as the C. pepofruit. Therefore, this study highlights the significance of exploring herbal remedies as a potential treatment option for inflammation-related diseases. |
